In Sam & Max Hit the Road, players take on the role of Sam, a talking dog, who runs a freelance police agency with his partner Max, a "rabbity thing". Sam & Max get called to the local carnival where they are hired to track down Trixie, a giraffe-necked singer, who has run off with the carnivals other main attraction Bruno, the Bigfoot. It seems, however, that Sam & Max are not the only ones trying to find Bruno; a country-western star called Conroy Bumpus is planning to add the Bigfoot to his menagerie.
As a result, Sam & Max set out on a trip all around the United States of America to track down the Bigfoot, preventing Bumpus from capturing the pair and eventually helping the Bigfoot species to return to their former glory.